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The exhaustive list of topics in Genetic Engineering And Applications in which we provide Help with Homework Assignment and Help with Project is as follows:

  • Role of genes within cells
  • Genetic code
  • Genetic elements that control gene expression
  • Method of creating recombinant DNA molecules
  • Vectors in recombinant DNA technology
  • Biology and salient features of vectors
  • Types of vectors - plasmids, cosmids, phages and viruses
  • Safety guidelines of recombinant DNA research
  • Restriction Endonculeases - Exo & exdo nucleases
  • Classification, mode of action. Enzymes in modification - Polynucleotide phosphorylase, DNase, Methylases, phosphatases, polynucleotide Kinase, Ligases, RNase and their mechansim of action
  • Nucleic Acid Hybridization And Amplification:
    • Methods of nucleic acid detection
    • Polymerase chain reaction (PCR) and its applications
    • Variations in PCR and applications
    • Methods of nucleic acid hybridization
    • Probe and target sequences
    • Southern and Northern hybridization techniques
    • Nucleic acid mutagenesis in vivo and in vitro
  • Construction Of DNA Libraries:
    • Isolation and purification of nucleic acids
    • Quantification
    • Storage
    • Isolation of plasmids
    • Construction of genomic and cDNA libraries
    • Screening and preservation
  • Gene Transfer Techniques:
    • Gene transfer techniques in plants, animals and microbes - Transformation
    • Electroporation
    • Microprojectile system
    • Liposome mediated transfer
    • Genegun
    • Agro bacterium-mediated gene transfer in plants - Tiplasmid: structure and functions
    • Ti plasmid based vectors - advantages
    • Chloroplast transformation
  • Transgenic Science And Genetic Improvement:
    • Transgenic science in plant improvement
    • Biopharming - plants as bioreactors
    • Transgenic crops for increased yield
    • Resistance to biotic and abiotic stresses
    • Techniques of gene mapping in plants
    • Restriction mapping
    • RFLP, RAPD, AFLP
    • Marker-assisted selection and breeding for improvement.
    • Transgenic science for animal improvement, biopharming - animals as bioreactors for recombinant proteins
    • Gene mapping in farm animals
    • Marker-assisted selection and genetic improvement of livestock
  • Microbial biotechnology - Genetic manipulation, engineering microbes for the production of antibiotics, enzymes, Insulin, growth hormones, monoclonal antibodies, clearing oil spills
  • Gene Therapy:
    • Methods of Gene therapy
    • Gene targeting and silencing
    • Gene therapy in the treatment of cancer
    • SCID
    • Muscular dystrophy
    • Respiratory disease (emphysema) 
    • Challenges in gene therapy
    • Future of gene therapy