The exhaustive list of topics in Biotechniques in which we provide Help with Homework Assignment and Help with Project is as follows:
- Techniques for DNA characterization: Construction of Genomic and cDNA library construction, Agarose gel electrophoresis, Northern blotting; Southern blotting, RFLP, AFLP, microarray.
- DNA manipulations techniques: PCR and its application, Restriction enzyme digestion, Ligation, Site directed mutagenesis.
- Enzymes in genetic engineering: DNA polymerases, Ligase Reverse transcriptase and Restriction endonucleases.
- Techniques for Protein characterization: SDS-Gel electrophoresis, Western blotting, IEF.
- 2D gel electrophoresis, Co-Immunoprecipitation, FRET, CHIP.
- Protein-ligand interactions, Affinity studies by Surface Plasmon resonance and Density gradient separation.
- Spectrophotometry (UV-Vis, CD, Fluorescence), Centrifugation, Radioactive isotopes and autoradiography, X-ray crystallography.
- Chromatography: Ion exchange; Gel filtration; Affinity; Reverse flow; HPLC.
- Immunological techniques: Generation of hybridoma generation and production of Antibodies, FACS, ELISA.
- Microscopy: light microscope, Fluorescence microscope, UV, Atomic absorption; Confocal).
- Cell culture and developmental biology techniques (FISH).
- Genetic crosses in model organism.