The exhaustive list of topics in Downstream Processing in which we provide Help with Homework Assignment and Help with Project is as follows:
- Bioprocess case studies/flow sheet
- Characteristics of biomolecules and their differences
- Various down stream process steps
- Fundamental principles of obtaining the product from cell cultures – intracellular vs. extracellular product
- Cell disruption – mechanical, enzymatic, and chemical methods
- Pre-treatment strategies
- Solid-liquid separation – filtration and centrifugation
- Adsorption
- Liquid-liquid extraction
- Membrane separation – ultrafiltration and reverse osmosis, dialysis.
- Precipitation of proteins by different methods
- Chromatograph – principles, instruments and practice
- Crystallization
- Distillation
- Drying
- lyophilization
- Stabilization of bioproducts
- Utilities (Air, water, steam etc)