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The exhaustive list of topics in Downstream Processing in which we provide Help with Homework Assignment and Help with Project is as follows:

  • Bioprocess case studies/flow sheet
  • Characteristics of biomolecules and their differences
  • Various down stream process steps
  • Fundamental principles of obtaining the product from cell cultures – intracellular vs. extracellular product
  • Cell disruption – mechanical, enzymatic, and chemical methods
  • Pre-treatment strategies
  • Solid-liquid separation – filtration and centrifugation
  • Adsorption
  • Liquid-liquid extraction
  • Membrane separation – ultrafiltration and reverse osmosis, dialysis.
  • Precipitation of proteins by different methods
  • Chromatograph – principles, instruments and practice
  • Crystallization
  • Distillation
  • Drying
  • lyophilization
  • Stabilization of bioproducts
  • Utilities (Air, water, steam etc)