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The exhaustive list of topics in Eukaryotic gene expression in which we provide Help with Homework Assignment and Help with Project is as follows:

  • CIS-ACTING ELEMENTS AND TRANS-ACTING FACTORS
  1. Eukaryotic RNA polymerases and basal transcription factors
  2. Diversity in core promoter elements
  3. Diversity in general transcription factors
  4. Proximal & Distal Promoter Elements, Enhancers and Silencers, Gene-specific Regulators
  • DOMAIN STRUCTURE OF EUKARYOTIC TRANSCRIPTION FACTORS
  1. Transcription factors – DNA binding domains
  2. Transcription factors – transcription activation domain
  • ROLE OF CHROMATIN IN EUKARYOTIC GENE REGULATION
  1. Role of chromatin in eukaryotic gene regulation
  2. Role of histones in eukaryotic gene regulation
  3. Role of DNA methylation in eukaryotic gene regulation
  4. Chromatin remodelling & gene regulation
  • SYNTHESIS OF mRNA, rRNA & tRNA
  1. mRNA processing – Role of RNA Pol II in mRNA capping  and mRNA splicing
  2. mRNA processing – Role of RNA Pol II in polyadenylation & mRNA editing
  3. Regulation of RNA Pol I transcription
  4. Regulation of RNA Pol III transcription
  • REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORS
  1. Signal Transduction Pathways - Introduction
  2. Regulation of gene expression by cyclicAMP
  3. Regulation of gene expression by second messengers other than cAMP
  4. Regulation of gene expression by Protein Kinase C
  5. Regulation of gene expression by Growth factors
  6. Regulation of gene expression by cytokines
  • REGULATION OF GENE EXPRESSION BY INTRACELLULAR RECEPTORS
  1. Regulation of gene expression by steroid hormones
  2. Regulation of gene expression by type II nuclear receptors
  3. Mechanism of transcriptional activation by nuclear receptors
  • REGULATION OF GENE EXPRESSION DURING DEVELOPMENT
  1. Gene Regulation during Drosophila Development
  2. Signal transduction pathways involved in embryonic development
  3. Homeotic genes
  4. Epigenetic regulation of gene expression during development
  5. Embryonic stem cells and Transcription factor-mediated epigenetic reprogramming
  • RECOMBINANT PROTEIN EXPRESSION SYSTEMS
  1. Cloning and Expression vectors
  2. Eukaryotic protein expression systems - I
  3. Eukaryotic protein expression systems - II
  4. Eukaryotic protein expression systems – III: Gene expression in mammalian cells using viral vectors
  • GENE THERAPY AND TRANSGENIC TECHNOLOGY
  1. Human Gene Therapy
  2. DNA vaccines
  3. Transgenic animals
  4. Transgenic plants
  5. Knockout mice
  •  EUKARYOTIC GENE EXPRESSION
  1. Regulation of Eukaryotic Gene Expression by Small RNAs (RNA Interference, RNAi)
  2. Genomics & Proteomics
  3. Metabolic Engineering & Synthetic Biology

 

  • CIS-ACTING ELEMENTS AND TRANS-ACTING FACTORS
  1. Eukaryotic RNA polymerases and basal transcription factors
  2. Diversity in core promoter elements
  3. Diversity in general transcription factors
  4. Proximal & Distal Promoter Elements, Enhancers and Silencers, Gene-specific Regulators
  • DOMAIN STRUCTURE OF EUKARYOTIC TRANSCRIPTION FACTORS
  1. Transcription factors – DNA binding domains
  2. Transcription factors – transcription activation domain
  • ROLE OF CHROMATIN IN EUKARYOTIC GENE REGULATION
  1. Role of chromatin in eukaryotic gene regulation
  2. Role of histones in eukaryotic gene regulation
  3. Role of DNA methylation in eukaryotic gene regulation
  4. Chromatin remodelling & gene regulation
  • SYNTHESIS OF mRNA, rRNA & tRNA
  1. mRNA processing – Role of RNA Pol II in mRNA capping  and mRNA splicing
  2. mRNA processing – Role of RNA Pol II in polyadenylation & mRNA editing
  3. Regulation of RNA Pol I transcription
  4. Regulation of RNA Pol III transcription
  • REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORS
  1. Signal Transduction Pathways - Introduction
  2. Regulation of gene expression by cyclicAMP
  3. Regulation of gene expression by second messengers other than cAMP
  4. Regulation of gene expression by Protein Kinase C
  5. Regulation of gene expression by Growth factors
  6. Regulation of gene expression by cytokines
  • REGULATION OF GENE EXPRESSION BY INTRACELLULAR RECEPTORS
  1. Regulation of gene expression by steroid hormones
  2. Regulation of gene expression by type II nuclear receptors
  3. Mechanism of transcriptional activation by nuclear receptors
  • REGULATION OF GENE EXPRESSION DURING DEVELOPMENT
  1. Gene Regulation during Drosophila Development
  2. Signal transduction pathways involved in embryonic development
  3. Homeotic genes
  4. Epigenetic regulation of gene expression during development
  5. Embryonic stem cells and Transcription factor-mediated epigenetic reprogramming
  • RECOMBINANT PROTEIN EXPRESSION SYSTEMS
  1. Cloning and Expression vectors
  2. Eukaryotic protein expression systems - I
  3. Eukaryotic protein expression systems - II
  4. Eukaryotic protein expression systems – III: Gene expression in mammalian cells using viral vectors
  • GENE THERAPY AND TRANSGENIC TECHNOLOGY
  1. Human Gene Therapy
  2. DNA vaccines
  3. Transgenic animals
  4. Transgenic plants
  5. Knockout mice
  •  EUKARYOTIC GENE EXPRESSION
  1. Regulation of Eukaryotic Gene Expression by Small RNAs (RNA Interference, RNAi)
  2. Genomics & Proteomics
  3. Metabolic Engineering & Synthetic Biology